Flow cytometry is a technology that is used to analyze the optical and fluorescence characteristics of particles as they pass through the laser beam. The cell components are fluorescently labelled and then excited by the laser to emit light at different wavelengths. The fluorescence can be measured to determine various properties of individual particles, which are usually cells. Properties measured include the particle’s relative granularity, size and fluorescence intensity as well as its internal complexity. Up to thousands of particles per second can be analyzed as they pass through the liquid stream, and an optical-to-electronic coupling system is used to record how the particle emits fluorescence and scatters incident light from the laser. Flow cytometer has numerous applications in science, including GFP expression analysis, ploidy analysis, immunophenotyping, cancer diagnosis, cell sorting, and cell counting.  |   https://www.creative-bioarray.com/support/principle-of-the-flow-cytometry.htm